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细胞性谷胱甘肽S-转移酶潜在抑制剂的分光光度筛选
JoVE 신문
생화학
Author Produced
This content is Free Access.
JoVE 신문 생화학
Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
DOI:

14:57 min

October 10, 2020

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Chapters

  • 00:00Introduction
  • 00:56Preparation of the Enzymatic Solution
  • 03:10Michaelis-Menten Constant of CDNB with GST
  • 04:42Absorbance of Potential GST Inhibitors
  • 06:08Determination of the IC50
  • 07:49Determination of the Type of Inhibition and Ki
  • 09:46Results: Testing the Potential of Curcumin as a GST Inhibitor
  • 13:56Conclusion

Summary

자동 번역

谷胱甘肽S-转移酶(GSTs)是参与许多化疗药物代谢的解毒酶。GSTs的表达与癌症化疗的抗性相关。对抗这种表型的一个方法就是使用抑制剂。该协议描述了一种使用分光光度测定来筛选潜在GST抑制剂的方法。

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