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聚焦超声诱导血脑屏障打开，用于靶向大脑结构和评估化学遗传神经调控
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JoVE 신문 신경과학
Focused Ultrasound Induced Blood-Brain Barrier Opening for Targeting Brain Structures and Evaluating Chemogenetic Neuromodulation
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聚焦超声诱导血脑屏障打开，用于靶向大脑结构和评估化学遗传神经调控

DOI:
10.3791/61352-v

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08:37 min

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December 22, 2020

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,
1Department of Bioengineering,Rice University

Chapters

  • 00:04Introduction
  • 00:54Animal Preparation
  • 02:30MRI‐Guided Targeting
  • 04:35Injection Solution Preparation
  • 05:59Insonation
  • 07:10Blood Brain Barrier (BBB) Opening Evaluation and Designer Receptors Activated Exclusively by Designer Drugs (DREADD) Stimulation
  • 07:47Results: BBB Opening and DREADD Expression Visualization
  • 08:11Conclusion

Summary

자동 번역

자동 번역

该协议描述了通过聚焦超声血脑屏障（BBB）开放进行基因传递所需的步骤，评估产生的基因表达以及通过组织学测试测量化学遗传受体的神经调控活性。

Tags

Focused UltrasoundBlood-brain BarrierNeuromodulationChemogenetic3D PrintedMRITargetingCatheterTail VeinAnesthesiaMouse

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