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Cancer Research

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JoVE Journal Cancer Research
Genoombrede analyse van DNA-methylatie bij maag- en darmkanker
 
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Genoombrede analyse van DNA-methylatie bij maag- en darmkanker

Article DOI: 10.3791/61355-v • 07:50 min • September 18th, 2020
September 18th, 2020 •
1,2, 2, 1,3, 3, 3, 2, 1
1Department of Surgery, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, 2Department of Coloproctological Surgery, Juntendo University Faculty of Medicine, 3Department of Surgery, Juntendo University Shizuoka Hospital

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Hierin beschrijven we een procedure voor genoombrede analyse van DNA-methylatie bij maag-darmkanker. De procedure is van belang voor studies die onderzoeken relaties tussen methylatiepatronen van genen en factoren die bijdragen aan carcinogenese bij maag-darmkanker.

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Kankeronderzoek maag-darmkanker DNA-methylatie genoombrede analyse tumor suppressor gen formaline-fixed paraffine-embedded sample DNA extraction bisulfite conversion macro-dissection quantitative methylation-specific PCR qMSP
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