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应用激光微分射发现人体肾脏组织中的区域转录学
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Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
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应用激光微分射发现人体肾脏组织中的区域转录学

DOI:
10.3791/61371-v

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05:46 min

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June 09, 2020

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, , , , , , , , , , , , ,
1Department of Medicine,Indiana University School of Medicine, 2Department of Cellular & Integrative Physiology,Indiana University School of Medicine, 3Division of Nephrology, Department of Medicine,Ohio State University Wexner Medical Center, 4Division of Pathology,Indiana University School of Medicine

Chapters

  • 00:05Introduction
  • 00:41Cryosectioning and Laser Microdissection (LMD)
  • 03:39Results: Representative Human Kidney Regional Transcriptomics Analyses
  • 05:05Conclusion

Summary

자동 번역

자동 번역

我们描述了人类肾脏的子段的激光微分射方案，包括球状、近肌管、厚升肢、收集管道和间质。然后从获得的隔间中分离RNA，进行RNA测序，以确定每个子段内转录签名的变化。

Tags

Laser MicrodissectionTranscriptomicsRegional AnalysisSingle Cell RNA SequencingCryosectioningImmunofluorescenceLaser Microdissection PlatformRNA IsolationLowly Expressed Transcripts

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