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恶性疟原虫 通过人工膜喂养进行配子体培养和蚊子感染

JoVE 신문
생물학

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JoVE 신문 생물학

Plasmodium falciparum Gametocyte Culture and Mosquito Infection Through Artificial Membrane Feeding

Please note that all translations are automatically generated. Click here for the English version.

恶性疟原虫通过人工膜喂养进行配子体培养和蚊子感染

DOI:

10.3791/61426-v

•

09:23 min

•

July 03, 2020

•

Abhai K Tripathi, Godfree Mlambo, Sachie Kanatani, Photini Sinnis, George Dimopoulos

¹Johns Hopkins Malaria Research Institute, W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health,Johns Hopkins University

Chapters

00:05Introduction
00:45P. falciparum Gametocyte Culture
02:23Standard Membrane Feeding Assay (SMFA)
04:57Midgut Dissection and Oocyst Load Quantification
06:01Salivary Gland Dissection and Sporozoite Load Quantification
07:00Results: Representative Gametocyte Culture Development
08:42Conclusion

Summary

자동 번역

对疟疾寄生虫的蚊子阶段进行详细调查对于设计有效的传播阻断策略至关重要。该协议展示了如何有效地培养传染性配子体，然后将这些配子体喂给蚊子以产生 恶性疟原虫的蚊子阶段。

Tags

Plasmodium Falciparum Gametocyte Culture Mosquito Infection Artificial Membrane Feeding Malaria Transmission Gametocytemia Exflagellation Anopheles Mosquitoes Blood Smear Centrifugation Cell Culture Microscopy

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