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Heterologöz β2-Microglobulin ile Yarasa Major Histocompatibility Complex Class I'in Stabilitesi ve Yapısı
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1School of Laboratory Medicine and Life Sciences,Wenzhou Medical University, 2NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention, 3Faculty of Health Sciences,University of Macau, 4CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology,Chinese Academy of Sciences, 5Savaid Medical School,University of Chinese Academy of Sciences
Chapters
- 00:04Introduction
- 00:54E. coli Transformation
- 01:46Culture Inoculation
- 02:59Bacteria Harvest
- 03:28Inclusion Body Purification
- 04:27MHC Complex Refolding
- 04:53MHC H Chain and Beta2m Injection and Dilution
- 05:42Refolded Protein Concentration Determination
- 06:51Crystallization, Data Collection, and Processing
- 07:41Structure Determination and Analyses
- 08:33Results: Representative Bat MHC I Beta2-Microglobulin Stability and Structure Analysis
- 10:21Conclusion
