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Nachweis von Phytophthora capsici im Bewässerungswasser mit Loop-Mediad Isothermale Amplifikation
 
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Nachweis von Phytophthora capsici im Bewässerungswasser mit Loop-Mediad Isothermale Amplifikation

Article DOI: 10.3791/61478-v • 07:25 min • June 25th, 2020
June 25th, 2020 •
1, 1, 2, 3, 4, 1
1Department of Plant Pathology, Coastal Plain Experiment Station, University of Georgia, 2University of Georgia Cooperative Extension, 3Department of Plant Pathology, Tropical Research & Education Center, University of Florida, 4Department of Agricultural and Environmental Sciences, Otis L. Floyd Nursery Research Center, Tennessee State University

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Wir haben eine Methode entwickelt, um Phytophthora capsici zoospores in Wasserquellen mit einer Filterpapier-DNA-Extraktionsmethode in Verbindung mit einem schleifenvermittelten isothermen Amplifikationstest (LAMP) zu detektieren, der im Feld oder im Labor analysiert werden kann.

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In diesem Monat in JoVE Ausgabe 160 Phytophthora capsici Bewässerungswasser Zoosporendetektion LAMP-Assay Filterpapier Schnellerkennung DNA-Extraktion In-Field-Diagnose
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