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JoVE Journal Medicine
Acceso a la citotoxicidad y respuesta celular a los biomateriales
 
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Acceso a la citotoxicidad y respuesta celular a los biomateriales

Article DOI: 10.3791/61512-v • 09:46 min • July 8th, 2021
July 8th, 2021 •
*1,2,3,4, *2,3,4,5, 1,2,3,4, 2,3,4,5, 2,3,4,6, 2,3,4,6, 2,3,4,7, 2,3,4,5, 1,2,3,4,8, 1,2,3,4
1Institute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 2Coimbra Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO), University of Coimbra, Coimbra, Portugal, 3Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Coimbra, Portugal, 4Clinical Academic Center of Coimbra, CACC, Portugal, 5Institute of Biophysics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 6Laboratory of Oncobiology and Hematology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 7Institute of Endodontics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 8Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal
* These authors contributed equally

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Esta metodología tiene como objetivo evaluar la citotoxicidad de biomateriales a través de la preparación de extractos solubles, utilizando ensayos de viabilidad y análisis fenotípicos, incluyendo citometría de flujo, RT-PCR, inmunocitoquímica y otras técnicas de biología celular y molecular.

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Retractación Número 173 biocompatibilidad biomateriales cultivo celular citotoxicidad medio acondicionado extractos
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