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Silicon Nanowires and Optical Stimulation for Investigations of Intra- and Intercellular Electrical Coupling
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Silicon Nanowires and Optical Stimulation for Investigations of Intra- and Intercellular Electrical Coupling

Silicon Nanowires and Optical Stimulation for Investigations of Intra- and Intercellular Electrical Coupling

DOI:
10.3791/61581-v

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08:58 min

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January 28, 2021

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, , ,
1The James Franck Institute,The University of Chicago, 2Department of Chemistry,The University of Chicago, 3The Institute for Biophysical Dynamics,The University of Chicago

Chapters

  • 00:04Introduction
  • 00:45Cell-Silicon Nanowire (SiNWs) Hybrid Preparation
  • 04:02Cardiomyocyte (CM):Myofibroblast SiNW Hybrid Co-Culture
  • 05:07Optical Imaging and Stimulation
  • 06:35Results: Representative Intra- and Intercellular Electrical Calcium Coupling Analyses
  • 08:30Conclusion

Summary

자동 번역

자동 번역

This protocol describes the use of silicon nanowires for intracellular optical bio-modulation of cell in a simple and easy to perform method. The technique is highly adaptable to diverse cell types and can be used for in vitro as well as in vivo applications.

Tags

Silicon NanowiresOptical StimulationIntracellular Bioelectrical InterrogationCell SpecificityCardiac CellsBrain CellsElectrical CommunicationMyofibroblastsCardiomyocytesChemical Vapor DepositionNanowire SuspensionCollagen Coating

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