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Chapters
- 00:04Introduction
- 00:57Bacterial Mating
- 02:47Generation of Final Bacterial Mutant Library
- 04:11DNA Shearing and Poly-C Tail Addition
- 06:23Results: Transposon Mutant Library
- 07:31Conclusion
We describe a method to generate saturating transposon mutant libraries in Gram-negative bacteria and subsequent preparation of DNA amplicon libraries for high-throughput sequencing. As an example, we focus on the ESKAPE pathogen, Acinetobacter baumannii, but this protocol is amenable to a wide range of Gram-negative organisms.
