Journal
/
생물학
/
Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging

Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging

DOI:
10.3791/61640-v

•

10:10 min

•

August 19, 2020

•

, , , , , , , ,
1Université Côte d’Azur,Inserm UMR1065, C3M, Team “Cellular and Molecular Pathophysiology of Obesity”, Nice, France, 2Integrated Cardio Metabolic Center, Department of Medicine,Karolinska Institutet, Stockholm, Sweden, 3Université Côte d’Azur,Inserm UMR1065, C3M, Team “Haematometabolism in Diseases”, Nice, France, 4Institut de Pharmacologie et de Biologie Structurale (IPBS),Université de Toulouse, CNRS, UPS, Toulouse, France

Chapters

  • 00:04Introduction
  • 00:35Mouse and Human White Adipose Tissue Fixation, Permeabilization, and Saturation
  • 02:59Mouse and Human White Adipose Tissue Staining
  • 05:28Mouse and Human White Adipose Tissue Clearing
  • 06:173D Confocal Imaging
  • 07:233D Adipose Tissue Image Quantitative Result Extraction
  • 07:57Results: Representative Adipose Tissue Labeling and Analysis
  • 09:34Conclusion

Summary

자동 번역

자동 번역

Here, we describe a simple, inexpensive and fast clearing method to resolve the 3D structure of both mouse and human white adipose tissue using a combination of markers to visualize vasculature, nuclei, immune cells, neurons, and lipid-droplet coat proteins by fluorescent imaging.

Tags

3D ImagingMethylsalicylate ClearingFormaldehyde FixationPBSGlycineTriton X-100DMSOTween 20DeoxycholateBSAPrimary Antibody Staining

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/61640?article_type=v

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
check_url/kr/61640?article_type=v

ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging
check_url/kr/61640?article_type=v

Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy
check_url/kr/61640?article_type=v

Adipo-Clear: A Tissue Clearing Method for Three-Dimensional Imaging of Adipose Tissue
check_url/kr/61640?article_type=v

Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining
check_url/kr/61640?article_type=v

Optical Clearing of Plant Tissues for Fluorescence Imaging
check_url/kr/61640?article_type=v

The Significance of Isolation, Culture, and Adipogenic Induction of SVF-Derived Preadipocytes from Mouse Perivascular Adipose Tissue (Video) | JoVE
check_url/kr/61640?article_type=v

Author Spotlight: Optimizing Supraclavicular Brown Adipose Tissue Extraction for Genetic Analysis
check_url/kr/61640?article_type=v

Murine Adipose Tissue Excision (Video) | JoVE

check_url/kr/61640?article_type=v

Isolation, Digestion, Filtration and Cell Plating of Murine Adipose Tissue (Video) | JoVE

Read Article