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准备和注射 Culex 蚊子的胚胎，使用CRISPR/Cas9产生空突变
 
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准备和注射 Culex 蚊子的胚胎，使用CRISPR/Cas9产生空突变

Article DOI: 10.3791/61651-v • 07:45 min • September 10th, 2020
September 10th, 2020 •
1, 2
1Department of Entomology, The Ohio State University, 2Insect Transformation Facility, Institute for Bioscience and Biotechnology Research, University of Maryland

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CRISPR/Cas9越来越多地用于非模型生物体的基因功能特征。该协议描述了如何产生 Culex皮皮恩斯的淘汰线，从准备注射混合，获得和注射蚊子胚胎，以及如何后方，交叉和屏幕注射蚊子及其后代所需的突变。

Tags

生物学， 第 163 期， 设计指南 RNA， 微操纵， 微注射， 蚊子胚胎， 突变筛选， 北方房子蚊子， 基因组编辑
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