Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing

Jia Wang; Dongsheng Xu; Jingjing Cui; Chen She; Hui Wang; Shuang Wu; Ling Zou; Jianliang Zhang; Wanzhu Bai

doi:10.3791/61742

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Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing

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Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing

DOI:

10.3791/61742-v

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07:28 min

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January 06, 2021

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Jia Wang*¹, Dongsheng Xu*¹, Jingjing Cui, Chen She, Hui Wang, Shuang Wu, Ling Zou, Jianliang Zhang, Wanzhu Bai

¹Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences

Chapters

00:04Introduction
00:45Innervation of Rat Cranial Dura Mater
02:34Retrograde Tracing Study with Fluorogold
05:20Results: Neurovascular Structure of the Cranial Dura Mater and Retrograde-labeled Neurons in the TG and DRGs
07:02Conclusion

Summary

자동 번역

Here we present a protocol to visualize spatial correlation of calcitonin gene-related peptide (CGRP)-immunoreactive nerve fibers and blood vessels in the cranial dura mater using immunofluorescence and fluorescent histochemistry with CGRP and phalloidin, respectively. In addition, the origin of these nerve fibers was retrograde traced with a fluorescent neural tracer.

Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing

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Chapters

Summary

자동 번역

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