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可视化与钙西通宁基因相关的肽免疫活性内活性与免疫发光和神经追踪

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Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing

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可视化与钙西通宁基因相关的肽免疫活性内活性与免疫发光和神经追踪

DOI:

10.3791/61742-v

•

07:28 min

•

January 06, 2021

•

Jia Wang*¹, Dongsheng Xu*¹, Jingjing Cui, Chen She, Hui Wang, Shuang Wu, Ling Zou, Jianliang Zhang, Wanzhu Bai

¹Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences

Chapters

00:04Introduction
00:45Innervation of Rat Cranial Dura Mater
02:34Retrograde Tracing Study with Fluorogold
05:20Results: Neurovascular Structure of the Cranial Dura Mater and Retrograde-labeled Neurons in the TG and DRGs
07:02Conclusion

Summary

자동 번역

在这里，我们提出了一个协议，可视化钙素基因相关肽（CGRP）免疫活性神经纤维和血管的空间相关性，分别使用CGRP和phalloidin的免疫荧光和荧光组织化学。此外，这些神经纤维的起源是逆行跟踪荧光神经示踪剂。

Tags

Keywords: CGRP Dura Mater Immunofluorescence Neural Tracing Cranial Nerves Blood Vessels Rat Confocal Microscopy Whole Mount Immunohistochemistry Fluorogold

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