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Neuroscience

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JoVE Journal Neuroscience
Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing
 

Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing

Article DOI: 10.3791/61742-v • 07:28 min • January 6th, 2021
January 6th, 2021 •
*1, *1, 1, 1, 1, 1, 1, 1, 1
1Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences
* These authors contributed equally

챕터

요약
Automatic Translation

Here we present a protocol to visualize spatial correlation of calcitonin gene-related peptide (CGRP)-immunoreactive nerve fibers and blood vessels in the cranial dura mater using immunofluorescence and fluorescent histochemistry with CGRP and phalloidin, respectively. In addition, the origin of these nerve fibers was retrograde traced with a fluorescent neural tracer.

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Keywords: CGRP Dura Mater Immunofluorescence Neural Tracing Cranial Nerves Blood Vessels Rat Confocal Microscopy Whole Mount Immunohistochemistry Fluorogold
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