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JoVE Journal Biology
Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors (FAPs) and Myogenic Progenitors (MPs) in Skeletal Muscle in the Rat
 

Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors (FAPs) and Myogenic Progenitors (MPs) in Skeletal Muscle in the Rat

Article DOI: 10.3791/61750-v • 09:49 min • June 9th, 2021
June 9th, 2021 •
*1, *1, 1, 1,2
1Keenan Research Center for Biomedical Science, St Michaels Hospital, Unity Health Toronto, 2Department of Medicine and Interdepartmental Division of Critical Care Medicine, University of Toronto
* These authors contributed equally

챕터

요약
Automatic Translation

This protocol outlines a method to isolate Fibro-adipogenic progenitors (FAPs) and myogenic progenitors (MPs) from rat skeletal muscle. Utilization of the rat in muscle injury models provides increased tissue availability from atrophic muscle for the analysis and a larger repertoire of validated methods to assess muscle strength and gait in free-moving animals.

Tags

Keywords: Fibro-Adipogenic Progenitors (FAPs) Myogenic Progenitors (MPs) Skeletal Muscle Rat Muscle Regeneration Fibrosis Tissue Processing Collagenase II Dispase Cell Strainer
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