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Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
JoVE 신문
생화학
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JoVE 신문 생화학
Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
DOI:

05:56 min

November 12, 2020

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Chapters

  • 00:05Introduction
  • 00:36Daily Checkpoint
  • 01:31Spot Size Calibration
  • 02:36svFCS Data Acquisition
  • 03:56Results: Representative svFCS Diffusion Law and Total Cholesterol Comparison
  • 05:07Conclusion

Summary

자동 번역

This article aims to present a protocol on how to build a spot variation Fluorescence Correlation Spectroscopy (svFCS) microscope to measure molecular diffusion at the plasma membrane of living cells.

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