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心脏球形作为体外生物工程心脏组织研究人类心脏病理生理学
JoVE 신문
생체공학
Author Produced
This content is Free Access.
JoVE 신문 생체공학
Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology
DOI:

10:41 min

January 23, 2021

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Chapters

  • 00:00Introduction
  • 00:54Cardiac Spheroid Formation
  • 05:46Drug Testing
  • 06:42Representative Results
  • 07:36Endothelial Cell Network Staining
  • 09:52Representative Results
  • 10:04Conclusion

Summary

자동 번역

该协议旨在通过在悬挂滴中共同培养细胞来制造3D心脏球体(CS)。胶原蛋白嵌入的 CS 在生理浓度下用多索鲁比辛(DOX,一种心毒性剂)进行治疗,以模拟心力衰竭。使用 DOX 治疗的 CS 进行体外测试可用于确定心力衰竭患者的新疗法。

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