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JoVE Journal Neuroscience
ROS Live Cell Imaging During Neuronal Development
 

ROS Live Cell Imaging During Neuronal Development

Article DOI: 10.3791/62165-v • 09:25 min • February 9th, 2021
February 9th, 2021 •
1,2, 1,2, 1,2,3
1Department of Biological Sciences, Purdue University, 2Purdue Institute for Integrative Neuroscience, Purdue University, 3Bindley Bioscience Center, Purdue University

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요약
Automatic Translation

This protocol describes the use of a genetically encoded hydrogen peroxide (H2O2)-biosensor in cultured zebrafish neurons and larvae for assessing the physiological signaling roles of H2O2 during nervous system development. It can be applied to different cell types and modified with experimental treatments to study reactive oxygen species (ROS) in general development.

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Keywords: ROS Live Cell Imaging Zebrafish Neurons Larvae Hydrogen Peroxide Biosensor Reactive Oxygen Species Neuronal Development Real-time Detection Rodent Model Coverslips Poly-D-Lysine Laminin Retinal Ganglion Cells RGCs Embryo Dissection ZFCM+ Media Zebrafish Embryos
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