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原发性小鼠视网膜色素上皮细胞的有效解剖与培养

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Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells

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原发性小鼠视网膜色素上皮细胞的有效解剖与培养

DOI:

10.3791/62228-v

•

08:33 min

•

February 10, 2021

•

Blanca Chinchilla, Heran Getachew, Rosario Fernandez-Godino

¹Ocular Genomics Institute of Massachusetts Eye and Ear,Harvard Medical School

Chapters

00:04Introduction
01:04Collection of RPE
04:09Isolation of Primary Mouse RPE Cells
06:37Results: Morphological and Functional Validation of RPE Cells
07:38Conclusion

Summary

자동 번역

该协议最初由费尔南德斯-戈迪诺等人于2016年1月报告，描述了一种有效分离和培养小鼠RPE细胞的方法，该细胞在一周内在Transwell板上形成功能和极化的RPE单层。手术大约需要3个小时。

Tags

Primary Mouse Retinal Pigment Epithelial Cells RPE Cell Culture Eye Dissection Age-related Macular Degeneration Hyaluronidase HBSS Buffer Cornea Incision Ora Serrata

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