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A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis
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A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis

A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis

DOI:
10.3791/62318-v

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08:06 min

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March 19, 2021

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, ,
1Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht

Chapters

  • 00:04Introduction
  • 00:50Generation of the Chip
  • 02:21Preparing Microfluidics Experiment
  • 03:44Microfluidics Experiment
  • 05:57Results: Entrainment Analysis Using Microfluidics Experiments
  • 06:58Conclusion

Summary

자동 번역

자동 번역

A protocol for the culture and manipulation of mouse embryonic tissue on a microfluidic chip is provided. By applying pulses of pathway modulators, this system can be used to externally control signaling oscillations for the functional investigation of mouse somitogenesis.

Tags

Keywords: MicrofluidicsSignaling OscillationsSomitogenesisSignaling DynamicsPDMSPlasma BondingFibronectin CoatingMouse EmbryosFunctional Dissection

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