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免疫荧光染色，用于 在果蝇 唾液腺中可视化异色素相关蛋白质
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Immunofluorescent Staining for Visualization of Heterochromatin Associated Proteins in Drosophila Salivary Glands
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免疫荧光染色，用于 在果蝇 唾液腺中可视化异色素相关蛋白质

DOI:
10.3791/62408-v

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10:13 min

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August 21, 2021

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, ,
1Instituto de Biotecnología, Departamento de Genética del Desarrollo y Fisiología Molecular,Universidad Nacional Autónoma de México

Chapters

  • 00:05Introduction
  • 01:01Third Instar Larvae Culture
  • 01:51Larvae Collection
  • 02:49Salivary Gland Tissue Fixation
  • 03:20Salivary Gland Tissue Wash
  • 03:55Permeabilization Step
  • 04:17Salivary Gland Tissue Wash
  • 04:55Tissue Blocking Step
  • 05:13Immunostaining
  • 08:01Representative Results
  • 09:50Conclusion

Summary

자동 번역

자동 번역

此协议旨在可视化 多聚 体细胞中的异色素聚合物。

Tags

Immunofluorescent StainingHeterochromatinDrosophilaSalivary GlandsPolytene CellsChromocenterThird Instar LarvaeFixationTris-Triton BufferBeta-mercaptoethanolBO3 BufferDTTBuffer B

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