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Nachweis der Proteinaggregation mittels Fluoreszenzkorrelationsspektroskopie
JoVE 신문
생물학
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JoVE 신문 생물학
Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
DOI:

14:04 min

April 25, 2021

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Chapters

  • 00:00Introduction
  • 01:10Sample Preparation #1- Cell Culture & Transfection
  • 02:51Sample Preparation #2- Cell Lysis & Medium Exchange
  • 04:28FCS Calibration
  • 08:48FCS Measurement
  • 11:45Representative Results
  • 13:03Conclusion

Summary

자동 번역

Wir führen hier ein Verfahren zur Messung von Proteinoligomeren und Aggregation in Zelllysat und lebenden Zellen mittels Fluoreszenzkorrelationsspektroskopie ein.

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