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穆林肺瓣膜相关成像的手术和样品处理

JoVE 신문
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Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve

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穆林肺瓣膜相关成像的手术和样品处理

DOI:

10.3791/62581-v

•

07:34 min

•

August 05, 2021

•

Yifei Liu, Yong-Ung Lee, Tai Yi, Ken Wu, Cedric Bouchet-Marquis, Han Chan, Christopher K. Breuer, David W. McComb

¹Center for Electron Microscopy and Analysis, The Department of Materials Science and Engineering,Ohio State University, ²Center for Regenerative Medicine,Nationwide Children’s Hospital, ³Thermo Fisher Scientific

Chapters

00:04Introduction
00:50Pulmonary Valve Excision
02:00Pressure Fixation of Pulmonary Valve
03:19En Bloc Sample Staining and Embedment
05:48Results: Correlative Imaging of the Murine Pulmonary Valve
07:10Conclusion

Summary

자동 번역

在这里，我们描述了一个相关的工作流程的切除，加压，固定和成像的粘液肺瓣，以确定总构象和局部细胞外矩阵结构。

Tags

Keywords: Murine Pulmonary Valve Correlative Imaging Structure-function Controlled Fixation High-resolution Imaging Mouse Dissection Thoracotomy Pulmonary Artery Pressurization Primary Fixative Solution

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