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Chapters
- 00:04Introduction
- 00:57Growing and Harvesting Yeast Cells
- 02:26Isolation of Plasma Membranes
- 04:14Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
- 06:22Results: Analysis of Cdr1-mGFPHis Isolated from Plasma Membranes of Saccharomyces cerevisiae
- 09:06Conclusion
This article presents a small-scale plasma membrane isolation protocol for the characterization of Candida albicans ABC (ATP-binding cassette) protein Cdr1, overexpressed in Saccharomyces cerevisiae. A protease-cleavable C-terminal mGFPHis double tag with a 16-residue linker between Cdr1 and the tag was designed to facilitate the purification and detergent-screening of Cdr1.
