/

/

CRISPR干扰（CRISPRI）在瘦素病原物种中基因沉默的应用

JoVE 신문
유전학
Author Produced

This content is Free Access.

JoVE 신문 유전학

Application of CRISPR Interference (CRISPRi) for Gene Silencing in Pathogenic Species of Leptospira

Please note that all translations are automatically generated. Click here for the English version.

CRISPR干扰（CRISPRI）在瘦素病原物种中基因沉默的应用

DOI:

10.3791/62631-v

•

14:49 min

•

August 14, 2021

•

L. G. V. Fernandes², R. L. Hornsby, A.L.T.O. Nascimento*², J. E. Nally*¹

¹Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service,United States Department of Agriculture, ²Laboratório de Desenvolvimento de Vacinas,Instituto Butantan

Chapters

00:04Introduction
02:56Protospacer Definition and Plasmid Construction
04:24Leptospira Transformation by Conjugation
09:35Colony Selection and Transconjugant Growth and Validation
13:01Representative Results
13:51Conclusions

Summary

자동 번역

在这里，描述了CRISPR干扰（CRISPRI）对瘦身物种中特定基因沉默的应用。瘦素细胞通过与表达 dCas9（催化"死亡"Cas9）的质粒结合而发生转化，以及单导RNA （sgRNA），负责与所需的基因组目标进行碱基配对。提出了验证基因沉默的方法。

Tags

Keywords: CRISPR Interference CRISPRi Gene Silencing Leptospira Pathogenic Species Hornsby-Alt-Nally Media Leptospirosis Genetic Tools DCas9 Single-guide RNA In Vitro Research Vaccine Development

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

选择依赖和独立生成CRISPR / Cas9介导的哺乳动物细胞基因敲除

CRISPR/Cas9 和 SsODN 在人细胞中催化点突变修复功能的标准方法研究

CRISPR/Cas9 蛋白注射到沟鲶、鮰松毛虫、基因编辑的胚胎

整合酶缺陷慢病毒载体载体的制备及其在细胞 CRISPR/Cas9-mediated 基因敲除中的表达

杆状病毒基因治疗应用的生产与纯化

CRISPR/Cas9 对小鼠和人造血祖细胞的高效基因破坏

CRISPR 的染色质环结构重组

CRISPR/Cas9 基因编辑制作人类疟疾寄生虫的条件突变体

基于多重 CRISPR 增强器的细胞系干扰增强功能解剖

使用基因激活 crispr 过度表达长非编码 rna

Read Article