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Congelación manual de muestras biológicas para microscopía electrónica criogénica de una sola partícula
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Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
DOI:

09:16 min

February 07, 2022

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Chapters

  • 00:04Introduction
  • 00:22Prepare the Manual Plunging Environment
  • 00:42Prepare the Cryogen Dewar and Manual Plunger
  • 02:04Prepare the Cryogen
  • 04:36Prepare Plunging Materials and Accessories
  • 05:13Prepare CryoEM Specimens by Plunge Freezing
  • 07:22Representative Results
  • 07:50Conclusion

Summary

자동 번역

Este manuscrito describe el método de blot-and-plunge para congelar manualmente especímenes biológicos para microscopía electrónica criogénica de una sola partícula.

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