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在斑马鱼胃中使用双光子显微镜进行深度和空间控制的体积消融

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Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula

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在斑马鱼胃中使用双光子显微镜进行深度和空间控制的体积消融

DOI:

10.3791/62815-v

•

09:50 min

•

July 15, 2021

•

Arthur Boutillon, Sophie Escot, Nicolas B. David

¹Laboratory for Optics and Biosciences,CNRS UMR7645, INSERM U1182, Institut Polytechnique de Paris

Chapters

00:05Introduction
00:38Preparation of the Two-photon Microscope
02:19Mounting the Embryo
03:46Locating the Embryo and Pre-Ablation Imaging
04:53Target Location, Laser Ablation and Post-ablation Verification
07:20Results: Analysis of the Zebrafish Embryo Before and Post Ablation
09:07Conclusion

Summary

자동 번역

胚胎发育需要细胞运动的大规模协调。双光子激发介导的激光烧蚀允许对大群深层细胞进行空间控制的三维烧蚀。此外，该技术可以探测体内集体迁移细胞对其机械环境中的扰动的反应。

Tags

Keywords: Two-photon Microscope Volume Ablation Zebrafish Gastrula Cell Interaction Cell Ablation Laser Power GFP MCherry Fluorescence Imaging Embryo Dechorionation Agarose Embedding

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