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Chapters
- 00:05Introduction
- 00:46Preparation of Stock Solutions and Tools
- 02:26Preparation and Dilutions of Peptides
- 04:18Preparing BSA-Peptide Gels
- 05:37Trimming, Processing, Embedding BSA Gels
- 07:00Pilot Evaluation of the Peptide Dilution Series and BSA Gel Tissue Microarray Construction
- 08:07Results: Staining of ERBB2/HER2 Peptide TMA with ERBB2/HER2 1+ and 3+ Cell Lines
- 08:46Conclusion
This work documents a simple method to create synthetic antigen controls for immunohistochemistry. The technique is adaptable to a variety of antigens in a wide range of concentrations. The samples provide a reference with which to assess intra- and inter-assay performance and reproducibility.
