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构建含有G蛋白偶联受体（GPCR）的脂质膜模型

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Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors (GPCRs)

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构建含有G蛋白偶联受体（GPCR）的脂质膜模型

DOI:

10.3791/62830-v

•

09:45 min

•

February 05, 2022

•

Ahmed Elbaradei*¹, Lucia Caterina Dalle Ore*¹, Noah Malmstadt

¹Mork Family Department of Chemical Engineering and Materials Science,University of Southern California

Chapters

00:05Introduction
01:16Preparation of Materials and Reagents
02:58Hydrogel Preparation
03:40Agarose and Protein Mixing
04:05Hydrogel and Lipid Deposition
06:09Swelling and Harvesting of Vesicles
08:03Representative Results: Microscopy of Vesicles
08:51Conclusion

Summary

자동 번역

该协议利用琼脂糖肿胀作为一种强大且可推广的技术，用于将整体膜蛋白（IMP）掺入巨型单层脂质囊泡（GUV）中，如本文所述，用于重建人1A 5-羟色胺受体蛋白（_5-HT1AR），这是药理学上重要的G蛋白偶联受体之一。

Tags

GUVs Giant Unilamellar Vesicles Membrane Proteins GPCRs G Protein-coupled Receptors 5-HT1AR Serotonin Receptor Hydrogel Scaffold Lipid Membrane Protein Incorporation Biomimetic Model In Vitro Studies

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