JoVE Journal
Immunology and Infection
Immunology and Infection
이 콘텐츠은(는) 무료 액세스입니다.
챕터
요약
This protocol has been established to culture tetrahydrobiopterin (BH4)- and inducible nitric oxide synthase (iNOS)-deficient primary murine macrophages to study NO-redox biology. The study focuses on reducing potential contamination of BH4 and other artifacts found in traditional isolation and culture methods which may confound experimental outcomes and interpretation of results.