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跨波森插入测序作为工具，以阐明细菌殖民因素在拉克霍尔德里亚角膜甲虫
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생물학
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JoVE 신문 생물학
Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles
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跨波森插入测序作为工具，以阐明细菌殖民因素在拉克霍尔德里亚角膜甲虫

DOI:
10.3791/62843-v

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09:55 min

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August 12, 2021

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, ,
1Department of Evolutionary Ecology, Institute of Organismic and Molecular Evolution,Johannes Gutenberg University, 2Department of Insect Symbiosis,Max Planck Institute for Chemical Ecology, 3Department of Plant and Environmental Sciences, Section for Organismal Biology,University of Copenhagen

Chapters

  • 00:04Introduction
  • 00:30Conjugation to Generate the Transposon Mutant Library
  • 03:32Mutant Pool Infection on Beetle Eggs
  • 04:43Sequencing Library Preparation
  • 08:34Results: Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors
  • 09:11Conclusion

Summary

자동 번역

자동 번역

这是一种适应方法，用于识别 伯克霍尔德里亚 有益共生的候选昆虫殖民因子。甲虫宿主感染了通过转波突变产生的随机突变库，殖民化后的库复杂性被比作 体外生长的对照。

Tags

Keywords: Transposon Insertion SequencingBurkholderia GladioliLagria Villosa BeetlesBacterial ColonizationGenetic ManipulationSymbiotic BacteriaConditionally Essential GenesE. Coli DonorBurkholderia Gladioli RecipientConjugationMutant Library

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