Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles

Ramya Ganesan; Martin Kaltenpoth; Laura V. Fl&#243;rez

doi:10.3791/62843

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Transposon-indsættelse Sekventering som et redskab til at belyse bakterielle kolonisering faktorer i en Burkholderia gladioli Symbiont af Lagria villosa Biller

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Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles

Transposon-indsættelse Sekventering som et redskab til at belyse bakterielle kolonisering faktorer i en Burkholderia gladioli Symbiont af Lagria villosa Biller

DOI:

10.3791/62843-v

•

09:55 min

•

August 12, 2021

•

Ramya Ganesan, Martin Kaltenpoth², Laura V. Flórez³

¹Department of Evolutionary Ecology, Institute of Organismic and Molecular Evolution,Johannes Gutenberg University, ²Department of Insect Symbiosis,Max Planck Institute for Chemical Ecology, ³Department of Plant and Environmental Sciences, Section for Organismal Biology,University of Copenhagen

Chapters

00:04Introduction
00:30Conjugation to Generate the Transposon Mutant Library
03:32Mutant Pool Infection on Beetle Eggs
04:43Sequencing Library Preparation
08:34Results: Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors
09:11Conclusion

Summary

자동 번역

Dette er en tilpasset metode til at identificere kandidat insekt kolonisering faktorer i en Burkholderia gavnlig symbiont. Billeværten er inficeret med et tilfældigt mutantbibliotek genereret via transposon mutagenese, og bibliotekets kompleksitet efter kolonisering sammenlignes med en kontrol dyrket in vitro.

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