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Coloration immunohistochimique fluorescente multiplexée de quatre types de cellules immunitaires de l’endomètre lors d’une fausse couche récurrente
 
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Coloration immunohistochimique fluorescente multiplexée de quatre types de cellules immunitaires de l’endomètre lors d’une fausse couche récurrente

Article DOI: 10.3791/62931-v • 05:16 min • August 4th, 2021
August 4th, 2021 •
1, 1,2, 1, 1, 3, 1, 1,4, 1,5, 3, 1
1Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, 2Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Prince of Wales Hospital, 3Department of Obstetrics and Gynaecology, Shenzhen Baoan Women’s and Children’s Hospital, Shenzhen University, 4School of Medicine, Faculty of Medicine and Health Sciences, Keele University, 5Li Ka Shing Institute of Health Sciences; School of Biomedical Sciences, The Chinese University of Hong Kong

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Malgré les progrès de l’immunohistochimie multiplexe et de l’imagerie multispectrale, caractériser la densité et le regroupement simultané des principales cellules immunitaires dans l’endomètre reste un défi. Cet article décrit un protocole de coloration multiplex détaillé et une imagerie pour la localisation simultanée de quatre types de cellules immunitaires dans l’endomètre.

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Biologie Numéro 174 Multiplex Fluorescence Cellules immunitaires Méthodes Coloration
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