Journal
/
암 연구학
/
Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research
JoVE 신문
암 연구학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 암 연구학
Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research

Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research

DOI:
10.3791/62940-v

•

08:30 min

•

July 12, 2021

•

, , , , , ,
1Department of Reproductive Endocrinology, Wagistrasse 14,University of Zurich and University Hospital Zurich, 2Department of Dermatology, Wagistrasse 18,University of Zurich and University Hospital Zurich, 3Department of Pharmacology and Chemical Biology,University of Pittsburgh

Chapters

  • 00:04Introduction
  • 00:49Spheroid Formation
  • 03:55Spheroid Immunohistochemistry Study
  • 05:24Live/Dead Staining in Spheroids
  • 05:56Results: Generation of Mammary Epithelial and Endothelial Cell Spheroids
  • 07:41Conclusion

Summary

자동 번역

자동 번역

Crosstalk between mammary epithelial cells and endothelial cells importantly contributes to breast cancer progression, tumor growth, and metastasis. In this study, spheroids have been made from breast cancer cells together with vascular and/or lymphatic endothelial cells and demonstrate their applicability as an in vitro system for breast cancer research.

Tags

Breast CancerCell Spheroids3D ModelMammary Epithelial CellsEndothelial CellsCancer Cell-endothelial Cell InteractionsProliferationMetastasisIn Vitro ModelCell DistributionDye StainingEnzymatic DetachmentCell CountingCell SuspensionCell Co-culture

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/62940?article_type=v

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures
check_url/kr/62940?article_type=v

Surgical Procedures and Methodology for a Preclinical Murine Model of De Novo Mammary Cancer Metastasis
check_url/kr/62940?article_type=v

A Portal Vein Injection Model to Study Liver Metastasis of Breast Cancer
check_url/kr/62940?article_type=v

Sectioning Mammary Gland Whole Mounts for Lesion Identification
check_url/kr/62940?article_type=v

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies
check_url/kr/62940?article_type=v

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
check_url/kr/62940?article_type=v

Rat Mammary Epithelial Cell Transplantation into the Interscapular White Fat Pad
check_url/kr/62940?article_type=v

Orthotopic Transplantation of Breast Tumors as Preclinical Models for Breast Cancer
check_url/kr/62940?article_type=v

In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells
check_url/kr/62940?article_type=v

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples

Read Article