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Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632
 

Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632

Article DOI: 10.3791/62978-v 06:47 min November 6th, 2021
November 6th, 2021

챕터

요약

Here we present a modified method for the isolation and culture of human gingival epithelial cells by adding the Rock inhibitor, Y-27632, to the traditional method. This method is easier, less time-consuming, enhances stem cell properties, and produces larger numbers of high-potential epithelial cells both for the laboratory and for clinical applications.

Tags

Keywords: Primary Human Gingival Epithelial Cells Isolation Culture Y-27632 Enzymatic Method Tissue Pretreatment Digestion Rho-associated Kinase (ROCK) Inhibitor Cell Growth Direct Explant Method Periodontal Tissue Oral Functions Biomaterials
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