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分析EGFR突变型NSCLC患者衍生类器官靶向治疗的敏感性

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Profiling Sensitivity to Targeted Therapies in EGFR-Mutant NSCLC Patient-Derived Organoids

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分析EGFR突变型NSCLC患者衍生类器官靶向治疗的敏感性

DOI:

10.3791/63039-v

•

08:52 min

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November 22, 2021

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Dora Barbosa Rabago^2,3, Collin M. Blakely², Franziska Haderk^2,3, Trever G. Bivona^2,3

¹Department of Medicine,University of California, San Francisco, ²Helen Diller Family Comprehensive Cancer Center,University of California, San Francisco, ³Department of Cellular and Molecular Pharmacology,University of California, San Francisco

Chapters

00:04Introduction
01:05Generating Single-Cell Suspension
02:38Cell Seeding
04:39Drug Treatment
05:37Readout by Luminescence-Based Survival Assay
06:52Results: The Analysis of Treatment Response of EGFR-Mutant NSCLC Organoid Model to Osimertinib
08:18Conclusion

Summary

자동 번역

该协议描述了NSCLC患者衍生类器官模型中靶向信号传导抑制剂药物敏感性的标准化评估。

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Keywords: Organoids EGFR-mutant NSCLC Targeted Therapies Matrigel Cell Culture Dissociation Trypsinization Centrifugation Cell Counting Cell Suspension 96-well Plate

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