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用组织培养幼苗制备和饲养轴性昆虫用于叶甲虫的宿主 - 肠道微生物群相互作用研究
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Preparing and Rearing Axenic Insects with Tissue Cultured Seedlings for Host-Gut Microbiota Interaction Studies of the Leaf Beetle
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用组织培养幼苗制备和饲养轴性昆虫用于叶甲虫的宿主 - 肠道微生物群相互作用研究

DOI:
10.3791/63195-v

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06:56 min

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October 08, 2021

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, , , ,
1State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences,Hubei University, 2Institute of Plant Protection,Wuhan Institute of Landscape Architecture, 3McKetta Department of Chemical Engineering,University of Texas at Austin

Chapters

  • 00:05Introduction
  • 00:54Insect Rearing
  • 01:43Germ-Free Poplar Culturing
  • 02:40Egg Surface Sterilization and Rearing Axenic Larvae
  • 03:47Verification of Axenic Larvae with Culture-Dependent Assays
  • 04:40Verification of Axenic Individuals with Culture-Independent Assays
  • 05:32Results: Confirmation of Efficacy of Gut Bacteria Elimination
  • 06:07Conclusion

Summary

자동 번역

자동 번역

为了获得轴性昆虫，其卵表面被灭菌，然后使用轴性叶子饲养孵化的幼虫。这种方法为抗焦虑昆虫的制备提供了一种有效的方法，而无需施用抗生素或开发人造饮食，这也可以应用于其他食叶昆虫。

Tags

Axenic InsectsGut MicrobiotaLeaf BeetlePlant SeedlingsAlpha-naphthalene Acetic AcidMS MediumSurface SterilizationGerm-freeArtificial DietInsect Rearing

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