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Biochemistry

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JoVE Journal Biochemistry
Reinigung von endogenen Drosophila-Transientenrezeptor-Potentialkanälen
 
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Reinigung von endogenen Drosophila-Transientenrezeptor-Potentialkanälen

Article DOI: 10.3791/63260-v • 08:39 min • December 28th, 2021
December 28th, 2021 •
*1, *1, 1, 1, 1, 1
1Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center
* These authors contributed equally

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Basierend auf dem Montagemechanismus des INAD-Proteinkomplexes wurde in diesem Protokoll eine modifizierte Affinitätsreinigung plus Wettbewerbsstrategie entwickelt, um den endogenen Drosophila TRP-Kanal zu reinigen.

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Biochemie Ausgabe 178 Proteinreinigung Drosophila TRP-Kanal INAD
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