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1Department of Mechanical Engineering,University of Michigan, Ann Arbor, 2John A. Paulson School of Engineering and Applied Sciences,Harvard University, 3Department of Cellular and Molecular Biophysics,Max Planck Institute of Biochemistry, 4Department of Biomedical Engineering,University of Michigan, Ann Arbor, 5Department of Biophysics,University of Michigan, Ann Arbor, 6Cellular and Molecular Biology Program,University of Michigan, Ann Arbor
Chapters
- 00:05Introduction
- 00:48Preparation of Oil-Lipid-Mixture
- 02:12Vesicle Generation
- 05:42Imaging and 3D image Reconstruction
- 06:53Results: Encapsulation of Actin Bundle Structures
- 07:08Conclusion
Dieser Artikel stellt eine einfache Methode zur schnellen Herstellung von riesigen unilamellären Vesikeln mit verkapselten zytoskelettalen Proteinen vor. Die Methode erweist sich als nützlich für die Bottom-up-Rekonstitution von Zytoskelettstrukturen in Enstrikt- und Zytoskelett-Membran-Interaktionen.
