Journal
/
생화학
/
Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy

DOI:
10.3791/63377-v

•

07:20 min

•

February 18, 2022

•

, ,
1Molecular Biology,Massachusetts General Hospital, 2Department of Genetics,Harvard Medical School

Chapters

  • 00:04Introduction
  • 01:05Microtubule Visualization via TIRF Microscopy
  • 01:58Microscope Settings
  • 03:17Generation of Surface-Immobilized Microtubule Bundles
  • 04:57Image Microtubule Dynamics
  • 05:53Results: TIRF Microscopy of Crosslinked and Single Microtubules
  • 06:35Conclusion

Summary

자동 번역

자동 번역

Here, a TIRF microscopy-based in vitro reconstitution assay is presented to simultaneously quantify and compare the dynamics of two microtubule populations. A method is described to simultaneously view the collective activity of multiple microtubule-associated proteins on crosslinked microtubule bundles and single microtubules.

Tags

MicrotubulesTIRF MicroscopyMulti-component ReconstitutionMicrotubule DynamicsMicrotubule SubpopulationsSingle MicrotubulesMicrotubule BundlesFluorescent LabelingExperimental ParametersImaging Setup

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/63377?article_type=v

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
check_url/kr/63377?article_type=v

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
check_url/kr/63377?article_type=v

Imaging of Extracellular Vesicles by Atomic Force Microscopy
check_url/kr/63377?article_type=v

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
check_url/kr/63377?article_type=v

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
check_url/kr/63377?article_type=v

Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy
check_url/kr/63377?article_type=v

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
check_url/kr/63377?article_type=v

Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues
check_url/kr/63377?article_type=v

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
check_url/kr/63377?article_type=v

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

Read Article