/
/
Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Introduction
- 01:00Murine Allergic Rhinitis Model Establishment
- 02:03Isolation of Mononuclear Cells from the Nasal Mucosa
- 04:52Surface Staining for Flow Cytometry Analysis
- 06:08Results: Ovalbumin-Induced Murine Model Development to Explore the Role of ILC2s in Allergic Rhinitis
- 07:27Conclusion
Group 2 innate lymphoid cells (ILC2s), implicated in type 2 inflammation, mainly participate in response to helminth infection, allergic diseases, metabolic homeostasis, and tissue repair. In this study, a procedure to isolate ILC2s from murine nasal mucosa and detect the expression of CD226 is demonstrated.
