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JoVE Journal Bioengineering
Entwicklung antiviraler Wirkstoffe mittels Oberflächenplasmonenresonanz
 
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Entwicklung antiviraler Wirkstoffe mittels Oberflächenplasmonenresonanz

Article DOI: 10.3791/63541-v • 13:00 min • June 14th, 2022
June 14th, 2022 •
1,2
1Department of Environmental Health Sciences, University of California, Los Angeles, 2Department of General Surgery, Medical University of Vienna

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Das vorliegende Protokoll beschreibt neue Werkzeuge für SPR-Bindungsassays zur Untersuchung der CV-N-Bindung an HA, S-Glykoprotein, verwandte Hybrid-Glykane und High-Mannose-Oligosaccharide. SPR wird verwendet, um das KD für die Bindung von entweder dimerem oder monomerem CV-N an diese Glykane zu bestimmen.

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Bioengineering Ausgabe 184 Cyanovirin-N Oberflächenplasmonresonanz Sättigungstransferdifferenz-NMR Hüllspitzen Glykan rekombinantes Glykoprotein
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