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通过质谱法对白细胞中的心磷脂进行指纹识别，以快速诊断巴特综合征
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Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome
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通过质谱法对白细胞中的心磷脂进行指纹识别，以快速诊断巴特综合征

DOI:
10.3791/63552-v

•

06:48 min

•

March 23, 2022

•

, , ,
1Medical School,Swansea University, 2Department of Basic Medical Sciences, Neuroscience and Sense Organs,University of Bari Aldo Moro

Chapters

  • 00:04Introduction
  • 00:53Isolation of Leukocytes by Dextran Sedimentation of Red Blood Cells
  • 02:57“Mini-Extraction” of Lipids from Isolated Leukocytes
  • 04:03Lipid Analysis by MALDI-TOF/MS
  • 05:00Results: Diagnosis of Barth Syndrome Using Leukocyte CL Fingerprints and (MLCL + CLi)/CLm Ratios
  • 06:06Conclusion

Summary

자동 번역

자동 번역

该协议显示了如何获得白细胞心磷脂的质谱“指纹”以诊断Barth综合征。单心磷脂与心磷脂比值升高的评估将 Barth 综合征患者与对照心力衰竭患者区分开来，敏感性和特异性为 100%。

Tags

Cardiolipin FingerprintingLeukocytesMass SpectrometryBarth SyndromeMonolysocardiolipinMALDI-TOFBlood Sample ProcessingOsmotic ShockLipid Extraction

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