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Immunology and Infection

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JoVE Journal Immunology and Infection
Kokultur von murinen Dünndarm-Epithelorganoiden mit angeborenen lymphatischen Zellen
 
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Kokultur von murinen Dünndarm-Epithelorganoiden mit angeborenen lymphatischen Zellen

Article DOI: 10.3791/63554-v • 08:22 min • March 23rd, 2022
March 23rd, 2022 •
*1,2, *1,2,3, 1, 1
1Centre for Host-Microbiome Interactions, King’s College London, 2Wellcome Trust Cell Therapies and Regenerative Medicine Ph.D. Programme, King’s College London, 3Present address: Wellcome Trust/Cancer Research UK Gurdon Institute, Cambridge University
* These authors contributed equally

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Dieses Protokoll bietet detaillierte Anweisungen zur Etablierung von murinen Dünndarmorganoiden, zur Isolierung angeborener Lymphzellen des Typs 1 aus der murinen Dünndarmlamina propria und zur Etablierung von 3-dimensionalen (3D) Kokulturen zwischen beiden Zelltypen, um bidirektionale Wechselwirkungen zwischen Darmepithelzellen und angeborenen lymphatischen Typ-1-Zellen zu untersuchen.

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Immunologie und Infektion Ausgabe 181
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