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光ピンセットとデフォーカス顕微鏡を用いた赤血球の粘弾性特性の定量的解析
JoVE 신문
생물학
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JoVE 신문 생물학
Quantitative Analysis of Viscoelastic Properties of Red Blood Cells Using Optical Tweezers and Defocusing Microscopy
DOI:

08:03 min

March 25, 2022

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Chapters

  • 00:04Introduction
  • 00:43Preparation of Sample Holders and Cell Cultures
  • 01:30OT‐Based Rheology Experiment and Analysis
  • 03:12Video and Contrast Image Acquisition
  • 05:40Soft Glassy Rheology Model and Experimental Analysis
  • 07:03Results: Characterizing RBC Surface from Values of K’ (ω), K” (ω), G’, and G”
  • 07:30Conclusion

Summary

자동 번역

ここでは、細胞のレオロジー特性を測定するための光ピンセットとデフォーカス顕微鏡に基づく統合プロトコルについて説明します。このプロトコルは、さまざまな生理病理学的条件下での赤血球の粘弾性特性の研究に広く適用できます。

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