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小规模提取 秀丽隐杆线虫 基因组DNA

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JoVE 신문 생물학

Small-Scale Extraction of Caenorhabditis elegans Genomic DNA

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小规模提取秀丽隐杆线虫基因组DNA

DOI:

10.3791/63716-v

•

06:40 min

•

June 07, 2022

•

Bhoomi Madhu, Mohammed Farhan Lakdawala, Tina L. Gumienny

¹Department of Biology,Texas Woman’s University

Chapters

00:04Introduction
00:51Single-Worm DNA Extraction
02:54DNA Extraction of a Few Individuals
04:33Results: Successful Use of the Extracted Caenorhabditis elegans DNA as PCR Template
05:44Conclusion

Summary

자동 번역

这里介绍的是使用市售组织试剂盒从一只或几只动物中直接和相对快速地分离 秀丽隐杆线虫 基因组DNA的描述。所得的gDNA制剂是PCR的合适模板。

Tags

Caenorhabditis Elegans Genomic DNA Extraction Small-scale Classroom 연구 PCR Lysis Program Extraction Solution Neutralization Solution L4 Adult Hermaphrodite Platinum Wire Pick Centrifugation

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