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标准化 体外 测定,以可视化和量化人中性粒细胞与 金黄色葡萄球菌 生物膜之间的相互作用
JoVE 신문
면역학 및 감염병학
This content is Free Access.
JoVE 신문 면역학 및 감염병학
Standardized In vitro Assays to Visualize and Quantify Interactions between Human Neutrophils and Staphylococcus aureus Biofilms
DOI:

07:28 min

June 08, 2022

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Chapters

  • 00:04Introduction
  • 00:54Preparation of In Vitro Biofilm
  • 02:34Measurement of ROS Production by Neutrophils
  • 04:14Imaging the Biofilm-Neutrophil Interactions
  • 05:31Results: Assessing Neutrophils and Biofilms Interactions via Standardized In Vitro Assays
  • 06:56Conclusion

Summary

자동 번역

本协议描述了中性粒细胞 - 生物膜相互作用的研究。 金黄色葡萄球菌 生物膜在体外建立并与 周血来源的人中性粒细胞一起孵育。量化中性粒细胞的氧化爆发反应,并通过显微镜确定生物膜内的中性粒细胞定位。

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