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Developmental Biology

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Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
 

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Article DOI: 10.3791/63778-v 10:41 min May 19th, 2022
May 19th, 2022

챕터

요약

The basement membrane is essential for tissue and organ morphogenesis during development. To better understand the mechanisms leading to proper placement of this structure, the protocol presented describes methods to visualize and characterize the intracellular trafficking and secretion of basement membrane proteins in epithelial cells using confocal and super-resolution microscopy.

Tags

Keywords: Confocal Microscopy Super-resolution Imaging Polarized Intracellular Trafficking Basement Membrane Proteins Drosophila Oogenesis Fixation Immunostaining Fluorescent Secondary Antibodies Airyscan Microscopy
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