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JoVE Journal Developmental Biology
Präparation und morphologische Analyse von Küken-Schädel-Neuralleistenzellkulturen
 
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Präparation und morphologische Analyse von Küken-Schädel-Neuralleistenzellkulturen

Article DOI: 10.3791/63799-v • 09:26 min • June 27th, 2022
June 27th, 2022 •
1, 2,3, 2,3, 2,3
1Department of Biology and Neuroscience Program, Hamline University, 2Department of Genetics, Cell Biology and Development, University of Minnesota, 3Developmental Biology Center, University of Minnesota

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Dieses vielseitige Protokoll beschreibt die Isolierung von primären Neuralleistenzellen (NCCs) durch die Exzision von kranialen Neuralfalten aus Hühnerembryonen. Nach der Beschichtung und Inkubation entstehen wandernde NCCs aus neuronalen Faltenexplantaten, was eine Beurteilung der Zellmorphologie und -migration in einer vereinfachten 2D-Umgebung ermöglicht.

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Developmental Biology Ausgabe 184
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